MassQC Help > Metrics > Chromatography > Peptides per Minute          
Peptides per Minute

Peptides per Minute is the rate that peptides are detected.

Higher numbers of peptides eluting per minute indicate better peak resolution.

The Peptides per Minute metric is calculated around the middle of the LC elution time. This is the time that the peptides are eluting the fastest so that the separation of peptides is most critical. The early and late elution times are excluded from the calculation of this metric.

Units:

Peptides/minute

Optimal:

Higher: Since this metric is measuring detected peptides, a higher rate means that the instrument is able to detect peptides which are eluting closer together.

The most reproducible LC-MS performance is when this metric remains in a narrow range over a series of runs. Wide variability in any metric means your process is not reproducible.

What the Metric Detects:

An increase in Peptides per Minutes means more material is eluting more quickly.

Related Metrics:

The Peptides per Minute metric is measured over the time span given by the Retention Spread metric. A high Peptides per Minute value and a low Retention Spread may indicate problems with the chromatography system that are causing peptides to co-elute.

A low value of Peptides per Minute may correlate to a low overall detection of Peptides.

Related Troubleshooting Topics:
Forum:

If you have questions, comments and/or insights about MassQC, you can share them on the MassQC Users Forum.

Search the MassQC Site and the Help Center: