MassQC Help > Metrics > MS2 > High Intensity ID Rate          
High Intensity ID Rate

High Intensity ID Rate is the percentage of the more intense MS1 peaks that get identified as a peptide.

The High Intensity ID Rate measures the efficiency of sampling and detection.

The High Intensity ID Rate is calculated from only the most intense MS1 peaks. The most intense peaks are those in the top 25% ranked by the MS1 maximum intensity. The metric is the percentage of these intense peaks that the search engine matches to a peptide.

High-intensity ID

Units:

Percent (%)

Optimal:

Higher: Higher indicates increased efficiency

The most reproducible LC-MS performance is when this metric remains in a narrow range over a series of runs. Wide variability in any metric means your process is not reproducible.

What the Metric Detects:

The High Intensity ID Rate tells what fraction of peaks with stronger signals are matched to peptides. If this metric is unusually low, that may mean that the higher intensity peaks are noise.

Related Metrics:

Whereas the High Intensity ID Rate measures how efficiently the high intensity MS1 ions are matched to peptides, the Low Intensity ID Rate does the same thing for the low intensity MS1 ions. In general the High Intensity ID Rate is expected to be larger than the Low Intensity ID Rate and it is worth investigating if it is not.

Related Troubleshooting Topics:
Forum:

If you have questions, comments and/or insights about MassQC, you can share them on the MassQC Users Forum.

Search the MassQC Site and the Help Center: