MassQC Help > FAQs > Cysteine Residues          
Cysteine Residues - An IonSource Tutorial
S-Carboxymethylation of the Amino Acid Residue Cysteine with the Alkylating Agent Iodoacetic Acid

Cysteine residues in proteins are of great interest to Proteomics researchers. These amino acids are reactive and form from di-sulfide bonds breaking apart. When a technician reduces these bonds with a reagent like Dithiothreitol (DTT), free Sulfhydryl groups become present and these can react with a variety of functional groups. Often, a digestion protocol will call for reduction of the di-sulfides and consequent akylation with a reagent such as Iodoactemide (or Iodoacetic Acid) to cap the -SH groups.

>>Read the entire tutorial at IonSource.com

IonSource is a great resource for more information on this procedure We invite you to visit the site and learn more about S-Carboxymethylation of the Amino Acid Residue Cysteine with the Alkylating Agent Iodoacetic Acid.

Forum

If you have questions, comments and/or insights about MassQC, you can share them on the MassQC Users Forum.

Search the MassQC Site and the Help Center